Regulation of Pattern Formation and Gene Amplification During Drosophila Oogenesis by the miR-318 microRNA
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Pattern formation during epithelial development requires the coordination of multiple signaling pathways. Here, we investigate the functions of an ovary-enriched miRNA, miR-318, in epithelial development during Drosophila oogenesis. miR-318 maternal loss-of-function mutants were female sterile and laid eggs with abnormal morphology. Removal of miR-318 disrupted the dorsal-anterior follicle cell patterning, resulting in abnormal dorsal appendages. miR-318 mutant females also produced thin and fragile eggshells, due to impaired chorion gene amplification. We provide evidence that the ecdysone signaling pathway activates expression of miR-318 and that miR-318 cooperates with Tramtrack69 (Ttk69) to control the switch from endocycling to chorion gene amplification during differentiation of the follicular epithelium. The multiple functions of miR-318 in oogenesis illustrate the importance of miRNAs in maintaining cell fate and promoting the developmental transition in the female follicular epithelium.
Original language | English |
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Journal | Genetics (Print) |
Volume | 200 |
Issue number | 1 |
Pages (from-to) | 255-265 |
Number of pages | 11 |
ISSN | 0016-6731 |
DOIs | |
Publication status | Published - May 2015 |
ID: 134713180