Simplified protocol for clinical-grade tumor-infiltrating lymphocyte manufacturing with use of the Wave bioreactor
Research output: Contribution to journal › Journal article › Research › peer-review
BACKGROUND AIMS: The high level of complexity of current Good Manufacturing Practice-compliant methods of manufacturing hampers rapid and broad application of treatment with tumor-infiltrating lymphocytes (TILs).
METHODS: To ensure higher applicability of TIL production to laboratory routine, a practical and simple protocol of TIL manufacturing with the use of a closed-system bioreactor was developed and implemented at our institution.
RESULTS: This protocol enabled significant work load reduction during the most labor-intense step of TIL expansion, and allowed generation of high-quality TIL products, which mediated clinical regression in patients with metastatic melanoma.
CONCLUSIONS: Implementation of simplified methods of TIL expansion will speed up dissemination of TIL methods worldwide and will increase patient access to this highly effective treatment.
| Original language | English |
|---|---|
| Journal | Cytotherapy |
| Volume | 16 |
| Issue number | 8 |
| Pages (from-to) | 1117–1120 |
| Number of pages | 4 |
| ISSN | 1465-3249 |
| DOIs | |
| Publication status | Published - Aug 2014 |
- Bioreactors, CD8-Positive T-Lymphocytes, Cell Proliferation, Cell- and Tissue-Based Therapy, Humans, Immunotherapy, Adoptive, Lymphocytes, Tumor-Infiltrating, Melanoma, Neoplasm Metastasis
Research areas
ID: 138382344