Comparison of open-flow microperfusion and microdialysis methodologies when sampling topically applied fentanyl and benzoic acid in human dermis ex vivo
Research output: Contribution to journal › Journal article › Research › peer-review
PURPOSE: The purpose of this study is to compare two sampling methods--dermal Open-Flow Microperfusion (dOFM) and dermal Microdialysis (dMD) in an international joint experiment in a single-laboratory setting. We used human ex-vivo skin and sampled topically administered Fentanyl and Benzoic Acid. The second purpose was to provide guidance to researchers in choosing the most efficient method for a given penetrant and give suggestions concerning critical choices for successful dermal sampling.
METHODS: The dOFM and dMD techniques are compared in equal set-ups using three probe-types (one dOFM probe and two dMD probe-types) in donor skin (n = 9)--27 probes of each type sampling each penetrant in solutions applied in penetrationchambers glued to the skin surface over a time range of 20 h.
RESULTS: Pharmacokinetic results demonstrated concordance between dOFM and dMD sampling technique under the given experimental conditions. The methods each had advantages and limitations in technical, practical and hands-on comparisons.
CONCLUSION: When planning a study of cutaneous penetration the advantages and limitations of each probe-type have to be considered in relation to the scientific question posed, the physico-chemical characteristics of the substance of interest, the choice of experimental setting e.g. ex vivo/in vivo and the analytical skills available.
Original language | English |
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Journal | Pharmaceutical Research |
Volume | 29 |
Issue number | 7 |
Pages (from-to) | 1808-20 |
Number of pages | 13 |
ISSN | 0724-8741 |
DOIs | |
Publication status | Published - Jul 2012 |
- Administration, Topical, Analgesics, Opioid, Benzoic Acid, Dermis, Equipment Design, Female, Fentanyl, Humans, Microdialysis, Perfusion, Skin Absorption
Research areas
ID: 120834823