In vivo imaging of extracellular matrix remodeling by tumor-associated fibroblasts

Research output: Contribution to journal › Journal article › Research › peer-review

  • Jean Y Perentes
  • Trevor D McKee
  • Carsten D Ley
  • Hannah Mathiew
  • Michelle Dawson
  • Timothy P Padera
  • Lance L Munn
  • Rakesh K Jain
  • Yves Boucher
Here we integrated multiphoton laser scanning microscopy and the registration of second harmonic generation images of collagen fibers to overcome difficulties in tracking stromal cell-matrix interactions for several days in live mice. We show that the matrix-modifying hormone relaxin increased tumor-associated fibroblast (TAF) interaction with collagen fibers by stimulating beta1-integrin activity, which is necessary for fiber remodeling by matrix metalloproteinases.
Original languageEnglish
JournalNature Methods
Volume6
Issue number2
Pages (from-to)143-5
Number of pages2
ISSN1548-7091
DOIs
Publication statusPublished - 2009

Bibliographical note

Keywords: Animals; Cell Line, Tumor; Cell Movement; Collagen; Extracellular Matrix; Fibroblasts; Humans; Image Enhancement; Integrin beta Chains; Mice; Mice, Transgenic; Microscopy, Confocal; Relaxin; Soft Tissue Neoplasms; Stromal Cells

ID: 18764243