An analysis of the binding characteristics of a panel of recently selected ICAM-1 binding Plasmodium falciparum patient isolates
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- Correction - An Analysis of the Binding of a panel of recently selected ICAM-1 binding Plasmodium falciparum patient isolates
Final published version, 120 KB, PDF document
- An Analysis of the Binding Characteristics of a Panel of Recently Selected ICAM-1 Binding Plasmodium falciparum Patient Isolates.
Final published version, 448 KB, PDF document
The basis of severe malaria pathogenesis in part includes sequestration of Plasmodium falciparum-infected erythrocytes (IE) from the peripheral circulation. This phenomenon is mediated by the interaction between several endothelial receptors and one of the main parasite-derived variant antigens (PfEMP1) expressed on the surface of the infected erythrocyte membrane. One of the commonly used host receptors is ICAM-1, and it has been suggested that ICAM-1 has a role in cerebral malaria pathology, although the evidence to support this is not conclusive. The current study examined the cytoadherence patterns of lab-adapted patient isolates after selecting on ICAM-1. We investigated the binding phenotypes using variant ICAM-1 proteins including ICAM-1Ref, ICAM-1Kilifi, ICAM-1S22/A, ICAM-1L42/A and ICAM-1L44/A using static assays. The study also examined ICAM-1 blocking by four anti-ICAM-1 monoclonal antibodies (mAb) under static conditions. We also characterised the binding phenotypes using Human Dermal Microvascular Endothelial Cells (HDMEC) under flow conditions. The results show that different isolates have variant-specific binding phenotypes under both static and flow conditions, extending our previous observations that this variation might be due to variable contact residues on ICAM-1 being used by different parasite PfEMP1 variants.
Original language | English |
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Article number | e111518 |
Journal | PLOS ONE |
Volume | 9 |
Issue number | 10 |
Number of pages | 8 |
ISSN | 1932-6203 |
DOIs | |
Publication status | Published - 2014 |
Bibliographical note
Correction: The affiliation for the seventh author is incorrect and the correct affiliation is not indicated. Saeed A. Al-Harthi is not affiliated with #3. The correct affiliation is: Department of Parasitology, Faculty of Medicine, Umm AL-Qura University, Makkah, Kingdom of Saudia Arabia. Published in: PLoS ONE vol. 11, issue 2.
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