C-di-GMP regulates antimicrobial peptide resistance in Pseudomonas aeruginosa
Research output: Contribution to journal › Journal article › Research › peer-review
Bis-(3'-5')-cyclic dimeric GMP (c-di-GMP) is an intracellular second messenger which controls the life styles of many bacteria. A high intracellular level of c-di-GMP induces a biofilm lifestyle, whereas a low intracellular level of c-di-GMP stimulates dispersal of biofilms and promotes a planktonic lifestyle. Here, we used expression of different reporters to show that planktonic cells (PCells), biofilm cells (BCells) and cells dispersed from biofilms (DCells) had distinct intracellular c-di-GMP levels. Proteomics analysis showed that the low intracellular c-di-GMP level of DCells induced the expression of proteins required for the virulence and development of antimicrobial peptide resistance in P. aeruginosa. In accordance, P. aeruginosa cells with low c-di-GMP levels were found to be more resistant to colistin than P. aeruginosa cells with high c-di-GMP levels. This contradicts the current dogma stating that dispersed cells are inevitably more susceptible to antibiotics than their sessile counterparts.
Original language | English |
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Journal | Antimicrobial Agents and Chemotherapy |
Volume | 57 |
Issue number | 5 |
Pages (from-to) | 2066-2075 |
Number of pages | 10 |
ISSN | 0066-4804 |
DOIs | |
Publication status | Published - 2013 |
ID: 44310769