Bacillus caldolyticus prs gene encoding phosphoribosyldiphosphate synthase
Research output: Contribution to journal › Journal article › Research › peer-review
The prs gene, encoding phosphoribosyl-diphosphate (PRPP) synthase, as well as the flanking DNA sequences were cloned and sequenced from the Gram-positive thermophile, Bacillus caldolyticus. Comparison with the homologous sequences from the mesophile, Bacillus subtilis, revealed a gene (gcaD) encoding N-acetylglucosamine-l-phosphate uridyltransferase upstream of prs, and a gene homologous to ctc downstream of prs. cDNA synthesis with a B. caldolyticus gcaD-prs-ctc-specified mRNA as template, followed by amplification utilising the polymerase chain reaction indicated that the three genes are co-transcribed. Comparison of amino acid sequences revealed a high similarity among PRPP synthases across a wide phylogenetic range. An E. coli strain harbouring the B. caldolyticus prs gene in a multicopy plasmid produced PRPP synthase activity 33-fold over the activity of a haploid B. caldolyticus strain. B. caldolyticus PRPP synthase was resistant to heat treatment at 70°C to a much higher extent than PRPP synthase from B. subtilis
Original language | English |
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Journal | Gene |
Volume | 176 |
Issue number | 1-2 |
Pages (from-to) | 73-79 |
ISSN | 0378-1119 |
DOIs | |
Publication status | Published - 1996 |
Bibliographical note
Author Keywords: N-Acetylglucosamine-l-phosphate uridyltransferase; cDNA; ctc; gcaD; Operon expression; Phosphoribosylpyrophosphate; Phosphoribosyl diphosphate; Thermophile; tms
ID: 2508365