Protocols for screening antimicrobial peptides that influence virulence gene expression in Staphylococcus aureus
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Compounds that inhibit virulence gene expression in bacterial pathogens have received increasing interest as possible alternatives to the traditional antibiotic treatment of infections. For the human pathogen Staphylococcus aureus, we have developed two simple assays based on reporter gene fusions to central virulence genes that are easily applicable for screening various sources of natural and synthetic peptides for anti-virulence effects. The plate assay is qualitative but simultaneously assesses the effect of gradient concentrations of the investigated compound, whereas the liquid assay is quantitative and can be employed to address whether a compound is acting on the central quorum sensing regulatory system, agr, that controls a large number of virulence genes in S. aureus.
Original language | English |
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Title of host publication | Antimicrobial Peptides : Methods and Protocols |
Editors | Paul R. Hansen |
Number of pages | 8 |
Volume | 1548 |
Publisher | Humana Press |
Publication date | 2017 |
Pages | 387-394 |
Chapter | 28 |
ISBN (Print) | 978-1-4939-6735-3 |
ISBN (Electronic) | 978-1-4939-6737-7 |
DOIs | |
Publication status | Published - 2017 |
Series | Methods in Molecular Biology |
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Volume | 1548 |
ISSN | 1064-3745 |
- Agr quorum sensing, Anti-virulence, Compound screening, Hla, Peptides, RNAIII, S. aureus, Spa, β-Galactosidase/β-lactamase reporter genes
Research areas
ID: 182092202