The determination and analysis of site-specific rates of mitochondrial reactive oxygen species production
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Mitochondrial reactive oxygen species (ROS) are widely implicated in physiological and pathological pathways. We propose that it is critical to understand the specific sites of mitochondrial ROS production and their mechanisms of action. Mitochondria possess at least eight distinct sites of ROS production in the electron transport chain and matrix compartment. In this chapter, we describe the nature of the mitochondrial ROS-producing machinery and the relative capacities of each site. We provide detailed methods for the measurement of H2O2 release and the conditions under which maximal rates from each site can be achieved in intact skeletal muscle mitochondria.
Original language | English |
---|---|
Title of host publication | The determination and analysis of site-specific rates of mitochondrial reactive oxygen species production |
Number of pages | 29 |
Volume | 526 |
Publication date | 2013 |
Pages | 189-217 |
Chapter | 12 |
DOIs | |
Publication status | Published - 2013 |
Series | Methods in Enzymology |
---|---|
ISSN | 0076-6879 |
ID: 48877321