Quantifying protein-protein interactions in the ubiquitin pathway by surface plasmon resonance.
Research output: Contribution to journal › Journal article › Research › peer-review
The commercial availability of instruments, such as Biacore, that are capable of monitoring surface plasmon resonance (SPR) has greatly simplified the quantification of protein-protein interactions. Already, this technique has been used for some studies of the ubiquitin-proteasome system. Here we discuss some of the problems and pitfalls that researchers should be aware of when using SPR analyses for studies of the ubiquitin-proteasome system.
Original language | English |
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Book series | Methods in Enzymology |
Volume | 399 |
Pages (from-to) | 164-77 |
Number of pages | 13 |
ISSN | 0076-6879 |
DOIs | |
Publication status | Published - 2005 |
Bibliographical note
Keywords: Protein Binding; Proteins; Surface Plasmon Resonance; Ubiquitin
ID: 6493104