The structurally effect of surface coated rhamnogalacturonan I on response of the osteoblast-like cell line SaOS-2
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Osseointegration is important when implants are inserted into the bone and can be improved by biochemical surface coating of the implant. In this paper enzymatically modified rhamnogalacturonan I (RG-I) from apple and lupin was used for biochemical coating of aminated surfaces and the importance of the quality of RG-I, the nature of the binding, the fine structure of RG-I, and its effect on SaOS-2 cell line cultured on coated surfaces was investigated. SaOS-2 cells are osteoblast-like cells and a well-established in vitro model of bone-matrix forming osteoblasts. Purification by gel filtration could remove small fragments of galacturonic acid (GalA) and binding studies showed that the purity of the RG-I molecules was important for the quality of the coating. The structure of RG-I and osteoblast-like cells' viability were positively correlated so that high content of 1,4-linked galactose (Gal) and a low content of arabinose in the RG-I molecules favored cell viability. These results indicate that coating of implants with RG-I affect osseointegration positively.
Original language | English |
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Journal | Journal of Biomedical Materials Research. Part A |
Volume | 102 |
Issue number | 6 |
Pages (from-to) | 1961-1971 |
Number of pages | 11 |
ISSN | 1549-3296 |
DOIs | |
Publication status | Published - 2014 |
ID: 47684963